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产品目录

兔抗β-Catenin抗体(β连环蛋白抗体)图片
产品货号:
YT686
中文名称:
兔抗β-Catenin抗体(β连环蛋白抗体)
英文名称:
β-Catenin Rabbit Polyclonal Antibody
产品规格:
50μl
发货周期:
1~3天
产品价格:
询价
本品为来源于兔的抗β连环蛋白抗体的多克隆抗体
宿主:兔
用途:WB,IHC,FC
交叉反应性:人小鼠大鼠
分子量:85KDa
推荐稀释比例:WB(1:1000),IHC(1:200),FC(1:50-1:100)
克隆性:多克隆抗体

About this Antibody
Name β-Catenin Rabbit Polyclonal Antibody
Category Polyclonal antibody(pAb); Primary antibody
Isotype IgG
Purification Peptide affinity purified
About the Immunogen
Immunogen This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to β-catenin.
Gene ID 1499
SwissProt P35222
Synonyms CTNNB; DKFZp686D02253; FLJ25606; FLJ37923; β-catenin; Beta-Catenin
Category Developmental Biology
Background β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.


注意事项:
1. 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
2. 回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
3. 提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希翼获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。

储存条件:-20℃,有效期一年。
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