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产品目录

重组小鼠CNTF图片
产品货号:
GS4570
中文名称:
重组小鼠CNTF
英文名称:
Recombinant Murine Ciliary Neurotrophic Factor
产品规格:
5μg
发货周期:
1~3天
产品价格:
询价
分子量:Approximately 22.5kDa,a single non-glycosylated polypeptide chain containing 197 amino acids.
储存条件:冷冻(-20℃)

概述

Ciliary neurotrophic factor(CNTF)is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations.CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture.Furthermore,the protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks.In addition,CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress.Recombinant murine CNTF containing 197 amino acids and it shares 82%and 95%a.a.sequence identity with human and rat CNTF.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格

外观Sterile Filtered White lyophilized(freeze-dried)powder.
纯度>95%by SDS-PAGE and HPLC analyses.
来源Escherichia coli.
生物活性Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30ng/ml,corresponding to a specific activity of>3.3×104 IU/mg.
成分Lyophilized from a 0.2μmfiltered concentrated solution in 2×PBS,pH 7.4,2%trehalose.
内毒素Less than 1 EU/μg of rMuCNTF as determined by LAL method.
复溶We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列AFAEQSPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNISLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LTLQVSAFAY QLEELMALLE QKVPEKEADG MPVTIGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HHMGISAHES HYGAKQM
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