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产品目录

BstV1I内切酶图片
产品货号:
GS2206
中文名称:
BstV1I内切酶
英文名称:
BstV1I
产品规格:
50U
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)

使用说明

At 37℃ activity is 10%from maximum.

技术规格

Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1μgof pBR322 DNA in 1hourat 55℃ in a total reaction volume of 50μl.
Recognition sitesGCAGC(N)8↑
来源Bacillus stearothermophilus V1
Assayed onpBR322 DNA
Optimal bufferG?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.)
Optimal temperature55℃
Storage conditions10mMTris-HCl(pH 7.5);200 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;50%glycerol.Store at -20℃.
LigationsAfter 3-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1μgof DNA with 2 u.a.of enzyme for 16hoursat 55℃.
Methlation effectMethylation sensitivity not tested
Thermal inactivationenzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible EndsBbvI,BseXI,Bst71I,R9896
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